Biology of Trichogramma marandobai and T. manicobai (Hymenoptera: Trichogrammatidae) in eggs of Erinnyis ello (Lepidoptera: Sphingidae)

ABSTRACT Parasitoids of the genus Trichogramma are promising for the biological control of insect pests in several crops, including cassava, which is severely attacked by Erinnyis ello L., 1758 (Lepidoptera: Sphingidae). Evaluating the biological aspects of these parasitoids to understand their dynamics is an important step towards the implementation of this control strategy in the field. Thus, our objective was to evaluate the biology of Trichogramma manicobai Brun, Moraes & Soares, 1984, and T. marandobai Brun, Moraes & Soares, 1986 in E. ello eggs. The parasitoids were obtained by collecting E. ello eggs from a commercial production of cassava, and the host's eggs were obtained from laboratory and greenhouse rearings. The average duration of a generation (T), net reproduction rate (R0), intrinsic rate of increase (rm), and the finite rate of increase (λ) were estimated, and from these, the fertility life table was calculated. The results indicated that T. marandobai has both higher net reproduction rate and a higher intrinsic rate of increase as well as requires less time to double its population than T. manicobai. Thus, T. marandobai has potential for natural and conservative biological control of E. ello. In addition, its potential in applied biological control should be evaluated through studies on the viability of its mass rearing in alternative hosts and its dispersion behavior in the field.

Molecular characterization and core collection evaluation of Manihot esculenta Crantz / Caracterização molecular e avaliação de coleção nuclear de Manihot esculenta Crantz

Cassava is one of the most important subsistence crops in tropical regions. It is necessary to preserve and to know the genetic diversity existent for the adequate use of genetic resources. The evaluation of genetic diversity among genotypes results in information about potential parents in breeding programs, allows duplicates identification, and facilitates germplasm exchange between research institutions. The objective of this study was to characterize the genetic diversity of cassava accessions of North Brazil region. A total of 106 accessions were analyzed using ten microsatellite markers. The genetic parameters estimated were: expected heterozygosity (HE), observed heterozygosity (HO) and polymorphic information content (PIC). Clustering was performed using the UPGMA and Neighbor-Joining (NJ) method. Bayesian analysis, analysis of principal coordinates and identification of a core collection were also used. The ten loci amplified 8,40 alleles on average. The average heterozygosity estimates were: HE = 0.71, HO = 0.58 and PIC = 0.72. Genetic distances ranged from 0.158 to 0.908. Six (5,66%) accesses were redundant. Clustering and dispersion analysis didn't differentiate bitter from sweet cassava, and there wasn't correlation between groups and collect origin. The core collection consisted of 22 individuals that represented 94% of total allelic diversity and 20,75% of the base collection. The results indicate high dissimilarity between the accessions and allowed the detection of redundant genotypes, showing the use of genetic markers as informative tools for the management of collections. (AU)

A mandioca é uma das mais importantes culturas de subsistência em países tropicais. É preciso conservar e conhecer a diversidade genética para o uso adequado dos recursos genéticos. A avaliação da diversidade genética entre os genótipos resulta em informações sobre potenciais genitores em programas de melhoramento, possibilita a identificação de duplicatas, além disso, facilita o intercâmbio de germoplasma entre instituições de pesquisa. O objetivo deste trabalho foi caracterizar a diversidade genética dos acessos de mandioca da Região Norte do Brasil. Foram analisados 106 acessos por meio de dez marcadores microssatélites. Os parâmetros de diversidade genética estimados foram: heterozigosidade esperada (HE), heterozigosidade observada (HO) e conteúdo de informação polimórfica (PIC). Agrupamentos foram realizados pelo método UPGMA e Neighbor-Joining (NJ). Utilizou-se também análises bayesianas, dispersão por coordenadas principais e a identificação de uma coleção nuclear. Os dez locos amplificaram 8,40 alelos em média. A média das estimativas de diversidade foram altas: HE = 0,71, HO = 0,58 e PIC = 0,72. As distâncias genéticas variaram de 0,158 a 0,908. Seis (5,66%) acessos estão redundantes. Os agrupamentos e análises de dispersão não evidenciaram distinção entre variedades bravas e mansas e não foi identificada estrutura genética correspondente a origem dos acessos. A coleção nuclear foi formada por 22 indivíduos, que representaram 94% da diversidade alélica total e 20,75% da coleção base. Os resultados indicam alta dissimilaridade entre os acessos e permitiram a detecção de genótipos redundantes, mostrando o uso de marcadores genéticos como ferramentas informativas para o manejo de coleções. (AU)

Actividad antiinflamatoria del extracto etanólico de las hojas de Manihot esculenta Crantz (yuca) en un modelo experimental de inflamación aguda / Anti-Inflammatory Activity Of The Ethnolic Extract Of The Leaves Of Manihot Esculenta Crantz (Yuca) In An Experimental Model Of Acute Inflammation

Introducción: Las hojas de Manihot esculenta Crantz (yuca) han sido usadas alrededor del mundo y a lo largo del tiempo con el fin de disminuir la respuesta antiinflamatoria. Objetivo: Evaluar la actividad antiinflamatoria del extracto etanólico de la hoja de Manihot esculenta crantz en ratas. Métodos: Se realizó un estudio tipo experimental. La población de estudio fueron 60 ratas albinas sp. Rattus novergicus, distribuidas en 4 grupos de 15 ratas cada uno. Se usó una solución homogénea de extracto etanólico de hojas de Manihot esculenta crantz para su administración vía intraperitoneal. Se utilizó carragenina como inductor inflamatorio, que fue administrado por vía intradérmica; para la medición del edema plantar, se hizo uso del Digital Water Plethysmometer (LE7500). Se administró al grupo control negativo solución de tween 80/agua (1:10) a dosis de 1ml/10g., al grupo control positivo betametasona 4mg/Kg y a los grupos de tratamiento 1 y 2 Manihot esculenta crantz, 2 mg/kg y 4 mg/kg, respectivamente. Se utilizó la prueba de ANOVA de 1 cola y la prueba post-hoc de Tukey, para las comparaciones entre los grupos. Resultados: En el 37,67% del grupo de tratamiento 2 se observó una reducción del edema a las 3 horas de administrar Manihot esculenta crantz (p <0,05). En otras mediciones se encontró una tendencia no significativa en ambos grupos de administración de Manihot esculenta crantz a la reducción del edema plantar. Conclusión: El extracto etanólico de Manihot sculenta a partir de la dosis de 4 mg/kg parece tener actividad antiinflamatoria en la reducción del edema plantar en el modelo animal utilizado.

Introduction: Manihot esculenta Crantz (yucca) leaves have been used around the world and over time in order to decrease the anti-inflammatory response. Objective: To evaluate the anti-inflammatory activity of the ethanolic extract of the Manihot esculenta Crantz leaf in rats. Methods: An experimental study was conducted. The study population included 60 albino rats sp. Rattus novergicus, distributed in 4 groups of 15 rats each. A homogeneous solution of ethanolic extract of Manihot esculenta Crantz leaves was used for intraperitoneal administration. Carrageenan was used as an inflammatory inducer that was administered intradermally; for the measurement of plantar edema, the Digital Water Plethysmometer (LE7500) was used. Tween 80 / water solution (1:10) was administered to the negative control group at a dose of 1mL./100g., To the betamethasone positive control group 4mg / Kg and to treatment groups 1 and 2 Manihot esculenta Crantz, 2 mg / kg and 4 mg / kg, respectively. The 1-tail ANOVA test and the Tukey post hoc test were used for comparisons between the groups. Results: In 37.67% of treatment group 2 a reduction in edema was observed 3 hours after administering Manihot esculenta Crantz (p <0.05). In both administration groups of Manihot esculenta Crantz there was a nonsignificant trend to reduce plantar edema with values close to significance. Conclusion: The Manihot esculenta Crantz ethanolic extract at a 4 mg / kg dose probably have anti-inflammatory activity in this animal model of acute inflamation.

Application of Photoshop in Measurement of Microsphere Diameter and Microstructure Drawing of Medicinal Materials / 中国实验方剂学杂志

<b>Objective::To explore the feasibility of Photoshop image processing software in the micro-spherical diameter measurement and identification of medicinal materials, in order to provide a new method for the measurement and mapping of traditional Chinese medicine. <b>Method::Photoshop (CS3 version and above) software was used to measure the diameter of 200 Notoginseng Radix et Rhizoma albumin microspheres in the same batch, digital microscopy and Photoshop image processing software was used to draw schematic diagrams of microstructures of Notoginseng Radix et Rhizoma medicinal powders and its four common pseudo-products and powders, namely Bletillae Radix, Alpiniae Officinarum Rhizoma, Curcumae Longae Rhizoma and <italic>Manihot esculenta,</italic> and make the identification table for classification and identification. <b>Result::The average diameter of the microspheres was (30.62±4.21)μm, and the diameter was mainly distributed in 20-40 μm. The average diameter of the microspheres was verified by laser particle size analyzer (30.18±4.67)μm. The differences between the two methods were not statistically significant. There were many starch granules in Notoginseng Radix et Rhizoma powder, with no calcium oxalate needle, oil cell, brown pigment and stone cell. Microscopic identification could be made for Notoginseng Radix et Rhizoma powder and 4 common counterfeits. <b>Conclusion::Photoshop image processing software measures the diameter of the microspheres with a high speed, high accuracy, simplicity, and low requirements for measuring instruments. It provides a new way to quickly measure the diameter of microspheres. Photoshop image processing software draws a schematic diagram of the microstructure, which is convenient and faster. The original morphology of the ground-reactive microstructure provides a new method for the microstructural drawing.

Agronomic and biochemical evaluation of cassava clones with roots that have pink pulp / Avaliação agronômica e bioquímica de clones de mandioca com polpa rosada

ABSTRACT: Sweet cassava breeding programs are focused on the development of bio fortified cultivars that combine significant amounts of carotenoids in their reserve roots with desirable agronomic. The objective of this research was to evaluate agronomic and biochemical traits in sweet cassava clones with roots that have pink pulp. The nine genotypes were evaluated in two seasons in a randomized block design with three replications. Among the evaluated clones, the following stood out: i) for the height of the first branch (390/08, 345/08 and the control IAC 576-70); ii) for plant height (390/08, 345/08 e 378/08); iii) for shoot weight without original steam cutting (390/08, 406/08, 378/08 e 341/08); iv) for the percentage of starch in roots (378/08, 413/08, 390/08 and the control IAC 576-70); and v) for the root yield (the control IAC 576-70 and 341/08, 390/08, 406/08 e 387/08). In the 2011/2012 season, all clones cooked within 30 minutes, indicating that they all have good culinary qualities. Regarding the total carotenoid content in the roots, the clones that stood out were 406/08 and 341/08. All clones evaluated had HCN content below 100 mg kg-1. Clones 341/08 and 406/08 have agronomic and biochemical potential for direct cultivation by producers in the Cerrado region of Central Brazil and / or for use as stock in sweet cassava breeding programs.

RESUMO: Os programas de melhoramento genético de mandioca de mesa estão focados no desenvolvimento de variedades biofortificadas que aliem aos caracteres agronômicos a presença de carotenoides nas raízes de reserva. Neste trabalho, objetivou-se avaliar características agronômicas e bioquímicas em clones de mandioca de mesa com polpa rosada. Os nove genótipos foram avaliados por duas safras em delineamento experimental de blocos casualizados com três repetições. Dentre os clones avaliados se destacaram: i) para altura da primeira ramificação (390/08, 345/08 e a testemunha IAC 576-70); ii) para altura da planta (390/08, 345/08 e 378/08); iii) para peso da parte aérea sem a cepa (390/08, 406/08, 378/08 e 341/08); iv) para porcentagem de amido nas raízes (378/08, 413/08, 390/08 e a testemunha IAC 576-70); v) para produtividade de raízes (IAC 576-70 e 341/08, 390/08, 406/08 e 387/08). Na safra 2011/2012, todos os clones cozinharam em até 30 minutos, indicando que todos apresentam boas qualidades culinárias. Com relação ao teor de carotenoides totais nas raízes, os clones que se destacaram foram 406/08 e 341/08. Todos os clones avaliados apresentaram teores de HCN nas raízes de reserva de mandioca, inferiores a 100 mg kg-1. Os clones 341/08 e 406/08 apresentam potencial agronômico e bioquímico para cultivo direto pelos produtores na região do Cerrado do Brasil Central e/ou para a utilização como genitores em programas de melhoramento genético de mandioca de mesa.

Efecto de la aplicación de un recubrimiento de almidón de yuca modificado por vía ácida sobre el tomate larga vida / Effect of the application of an acid-modified cassava starch coating on the long-life tomato

RESUMEN Se buscó minimizar el efecto de factores ambientales sobre la calidad pos cosecha del tomate, mediante la aplicación de recubrimiento elaborado con almidón de yuca modificado por vía ácida. La modificación de la estructura original de una suspensión de almidón de yuca variedad SM 707-17, se realizó mediante adición de ácido cítrico, en concentración de 15% p/p respecto al almidón en base seca, bajo condiciones de temperatura y de velocidad de agitación controladas. El efecto de este recubrimiento, se comparó con el efecto de la aplicación de otros elaborados con base en almidón modificado enzimáticamente y almidón nativo. Se midieron las variables: sólidos solubles totales, firmeza, pH, acidez titulable, tasa de respiración, pérdida de peso y color durante 15 días, aplicando un diseño factorial completamente al azar 4x6. Los resultados, se analizaron mediante el software SPSS versión 19, con el cual, se encontró que el factor "Recubrimiento" no influyó sobre las variables de respuesta analizadas, a diferencia del factor "Tiempo", que tuvo efecto decisivo sobre las mismas.

ABSTRACT The aim was to minimize the effect of environmental factors on postharvest of tomato quality by applying a coating made with cassava starch modified by acid route. The modification of the original structure of a suspension of native starch of cassava variety SM 707-17, was carried out by addition of citric acid in concentration of 15% w/w with respect to the starch in dry base, under controlled temperature and speed of controlled agitation. The effect of this coating was compared with the effect of the application of others made on the basis of enzymatically modified starch and native starch. The variables total soluble solids, firmness, pH, titrable acidity, respiration rate, weight loss and color for 15 days were measured, applying a completely randomized 4x6 factorial design. The results were analyzed using SPSS software version 19, which found that the "Coating" factor did not influence the response variables analyzed, unlike the "Time" factor that had a decisive effect on them.

Cassava biopolymer polyamide hydrogel accelerates healing of induced skin woundsin rats / Hidrogel de biopolímero de poliamido de mandioca acelera o processo de cicatrização em feridas cutâneas induzidas em ratos

This study aimed to macroscopically and microscopically evaluate the healing of skin wounds induced in rats by topical application of cassava polyamide biopolymer hydrogel. In total, 32 rats were used and divided into four groups (n= 8): negative control - saline solution; positive control - use of commercial ointment; experimental group - I - ointment + cassava hydrogel; experimental group - II - cassava hydrogel. A 1cm2 wound induced on the animals dorsum was treated and evaluated. At day 21 post-operation, the animals were sacrificed by anesthetic overdose, and then 1cm2 of cicatricial skin from the wound region was collected. The material was cut to evaluate healing. In the macroscopic evaluation, complete healing was observed at the end of 21 days. Re-epithelialization was observed histologically; the connective tissue in the negative control, positive, and experimental - I groups was characterized by an abundance of collagen fibers, fibroblasts, and blood vessels. In experimental group - II additional healing was observed, as evidenced by the arrangement of collagen fibers and fibroblasts, and the reduction of neoformed vessels. Thus, we concluded that the hydrogel can assist in healing skin wounds, especially in the remodeling phase.(AU)

O objetivo deste estudo foi avaliar macro e microscopicamente a cicatrização de feridas cutâneas induzidas em ratos, a partir da aplicação tópica do hidrogel de biopolímero de poliamido de mandioca. Trinta e dois ratos foram divididos em quatro grupos (n= 8): controle negativo, tratado com solução salina; controle positivo, com pomada comercial; grupo experimental - I, com pomada + hidrogel de mandioca; grupo experimental - II, com hidrogel de mandioca. Feridas induzidas de 1cm 2 no dorso dos animais foram tratadas e avaliadas em intervalos de três a quatro dias. No 21º dia do pós-operatório, os animais foram mortos por aprofundamento anestésico, em seguida foi coletado 1cm 2 de pele da região cicatricial. O material foi cortado, corado pelas técnicas de hematoxilina-eosina e azocarmine-G, para avaliação da cicatrização. Na avaliação macroscópica, foi observada cicatrização completa no final do período de 21 dias. Histologicamente, observou-se reepitelização, o tecido conjuntivo no grupos controle negativo, positivo e experimental - I se caracterizou pela abundância de fibras colágenas, fibroblastos e vasos sanguíneos. No grupo experimental - II, a cicatrização sugere avanço de etapas, evidenciado pelo arranjo das fibras colágenas, pela redução de fibroblastos e dos vasos neoformados. Assim, foi possível concluir que o hidrogel de biopolímero de amido de mandioca pode auxiliar na cicatrização de feridas cutâneas, principalmente na fase de remodelação.(AU)

In vitro multiplication of cassava varieties / Multiplicação in vitro de variedades de mandioca / Multiplicación in vitro de variedades de mandioca

The implantation of a model of sustainable development for agriculture can happen with the contribution of the mandiocultura. But for this, culture needs to be strengthened. In vitro propagation is an instrument for this purpose. Micropropagation can provide growers with large quantities of vigorous and healthy cassava seedlings in a short time. The objective of this study was to evaluate the in vitro establishment of four varieties of cassava cultivated in the municipality of Colorado do Oeste, State of Rondônia, popularly known as Arara, Caturra, Cacau Vermelha and Roxinha. For that, an experiment was carried out in the Laboratory of In Vitro Cultivation at the Pole of Technological Innovation of the University of Cruz Alta (UNICRUZ), with a completely randomized design in a 4 x 2 factorial scheme, with 6 replications. The treatments consisted of explants grown in Murashige and Skoog (MS) medium without the presence of growth regulator and MS medium supplemented with of 6-benzylaminopurine (BAP). The results indicate that the mean contamination percentage of the explants was 47.19%, differing among the varieties. The best growth response in culture media, in the multiple comparison of means (Scott-Knott's test, 5%), was obtained with MS medium without BAP addition, with significant difference between varieties. Under the conditions of this experiment, it was evidenced that micropropagation is a viable tool for obtaining varieties of interest, with desired phytosanitary qualities, with varietal and large-scale authenticity.(AU)

A implantação de um modelo de desenvolvimento sustentável para a agricultura pode acontecer com a contribuição da mandiocultura. Mas para isso, a cultura precisa ser fortalecida. A propagação in vitro é um instrumento para este fim. A micropropagação pode proporcionar aos produtores grande quantidade de mudas de mandioca vigorosas e sadias em um curto espaço de tempo. O objetivo deste trabalho foi avaliar o estabelecimento in vitro de quatro variedades de mandioca cultivadas no município de Colorado do Oeste, Rondônia, popularmente conhecidas como Arara, Caturra, Cacau Vermelha e Roxinha. Para isso, foi realizado um experimento no Laboratório de Cultivo In Vitro no Polo de Inovação Tecnológica da Universidade de Cruz Alta (UNICRUZ), com delineamento inteiramente casualizado em esquema fatorial 4 x 2, com 6 repetições. Os tratamentos consistiram em explantes cultivados em meio Murashige e Skoog (MS) sem a presença de regulador de crescimento e meio MS suplementado com 6-benzilaminopurina (BAP). Os resultados indicam que a porcentagem média de contaminação dos explantes foi de 47,19%, diferindo entre as variedades. A melhor resposta de crescimento em meios de cultura, na comparação múltipla de médias (teste de Scott-Knott, 5%), foi obtida com meio MS sem adição de BAP, com diferença significativa entre as variedades. Nas condições deste experimento, ficou evidenciado que a micropropagação é uma ferramenta viável para obtenção de variedades de interesse, com qualidades fitossanitárias desejadas, com autenticidade varietal e em larga escala.(AU)

La implantación de un modelo de desarrollo sostenible para la agricultura puede suceder con el aporte de la mandiocultura. Pero, para eso, la cultura necesita ser fortalecida. La propagación in vitro es un instrumento para ese fin. La micropropagación puede proporcionar a los cultivadores grandes cantidades de plántulas de mandioca vigorosas y saludables en poco tiempo. El objetivo de esta investigación ha sido evaluar el establecimiento in vitro de cuatro variedades de mandiocas cultivadas en el municipio de Colorado del Oeste, Rondônia, popularmente conocidas como Arara, Caturra, Cacau Vermelha y Roxinha. Para eso, se realizó un experimento en el Laboratorio de cultivo in vitro en el Polo de Innovación Tecnológica de la Universidad de Cruz Alta (UNICRUZ), con delineamiento completamente casualizado en esquema factorial 4 x 2, con 6 repeticiones. Los tratamientos consistieron en explantes cultivados en medio Murashige y Skoog (MS) sin la presencia de regulador de crecimiento y medio MS suplementado con 6-bencilaminopurina (BAP). Los resultados indican que el porcentaje de contaminación promedio de los explantes fue de 47.19%, diferenciándose entre las variedades. La mejor respuesta de crecimiento en medios de cultivo, en la comparación múltiple de medias (prueba de Scott-Knott, 5%), se obtuvo con medio MS sin adición de BAP, con una diferencia significativa entre las variedades. Bajo las condiciones de este experimento, se evidenció que la micropropagación es una herramienta viable para obtener variedades de interés, con cualidades fitosanitarias deseadas, con autenticidad varietal y de gran escala.(AU)

Population Structure and Genetic Diversity of Sweet Cassava Accessions from the Midwestern, Southeastern and Southern Regions of Brazil

Abstract The objective of this work was to screen sweet cassava accessions collected in smallholding areas in the Midwestern, Southeastern and Southern regions of Brazil, using 15 SSR molecular markers, to determine population structure and genetic diversity. Polymorphism was detected in every loci analyzed, with mean of 6.33 alleles per locus, and mean polymorphism information content (PIC) of 0.6057, pointing out that the primers were highly informative. The observed heterozygosity ranged from 0.0709 (SSRY 101) to 0.9398 (GA 12), with a mean of 0.6511, and mean genetic diversity of 0.6578, ranging from 0.3592 (GA 136) to 0.8116 (SSRY 21). The most dissimilar combinations observed were BGM526PR-BGM596MS, BGM526PR-BGM622MS and BGM526PR-BGM629MS. The traditional cassava cultivars assessed were divided into four distinct groups: two with cultivars from the South, one from the Southeast and one from the Midwestern region of Brazil. The variances among and within groups determined by the analysis of molecular variance were 44 and 56%, respectively. The PhiPT parameter (analogue to Fst) of 0.44 indicates high differentiation among groups. Broad genetic diversity was found among the traditional sweet cassava cultivars assessed, and the most divergent groups were formed by cultivars from the South and the Midwestern regions of Brazil.

Proline synthesis and physiological response of cassava genotypes under in vitro salinity / Síntese de prolina e resposta fisiológica de genótipos de mandioca sob salinidade in vitro

ABSTRACT: The objective of this research was to evaluate the proline synthesis and physiological response of cassava genotypes which were micro propagated and induced to salinity stress in vitro. Micro cuttings of approximately 1.0cm long with a single bud of genotypes TBRS Tapioqueira, BRS Verdinha and Lagoão which were previously established in vitro were inoculated in a MS medium containing different concentrations of NaCl (0; 25; 50; 75; 100mM) and were analyzed after 90th day for: number of roots, number of leaves and shoot dry mass. The proline content of BRS Tapioqueira and Lagoão was assessed at 30th, 60th and 90th day. There was no analysis of proline of the variety Verdinha because of the contamination of the explants. The experimental design was completely randomized in double factorial scheme (3 genotypes x 5 salt treatments), with seven repetitions for growth variables. For comparing proline content, completely randomized design was used in a plot subdivided in time, with genotype and NaCl factors in plot and time in subplot, with two repetitions. For r time and genotypes Tukey test (P<0,05) was used and for NaCl levels regression test (P<0,05). Salinity affected the growth of all varieties; although, BRS Tapioqueira and BRS Verdinha were less affected by induced salt stress. There was an increase in the accumulation of proline from the salt increment, this synthesis of proline being a biochemical indicator of salt stress in cassava plants cultivated in vitro.

RESUMO: O objetivo deste trabalho foi avaliar a síntese de prolina e respostas fisiológicas de variedades de mandioca micropropagadas e induzidas ao estresse salino in vitro. Microestacas das variedades BRS Tapioqueira, BRS Verdinha e Lagoão previamente estabelecidas in vitro foram inoculadas em meio MS com diferentes concentrações de NaCl (0; 25; 50; 75; 100mM) e aos 90 dias foram analisados: número de raiz, número de folhas e massa seca de parte aérea. O teor de prolina das variedades BRS Tapioqueira e Lagoão foi analisado aos 30, 60 e 90 dias. Não houve análise de prolina da variedade Verdinha por causa da contaminação dos explantes. O delineamento experimental foi inteiramente casualizado em esquema fatorial 3 genótipos x 5 tratamentos salinos, com sete repetições para as variáveis de crescimento. Para o conteúdo de prolina foi considerado inteiramente casualizado subdividido no tempo, com genótipos e NaCl na parcela e o tempo na subparcela e duas repetições. Para os fatores variedade e tempo, foi utilizado o teste de Tukey (P<0,05) e para tratamentos salinos, teste de Regressão (P<0,05). A salinidade afetou o crescimento de todas as variedades, porém BRS Tapioqueira e BRS Verdinha mostraram-se menos afetadas pelo estresse salino induzido. Houve aumento no acúmulo de prolina a partir do incremento de sal, sendo então este, um indicador bioquímico de estresse salino em plantas de mandioca cultivadas in vitro.

Thermal Inactivation of Crude Polyphenol Oxidases and Peroxidase from Three Tissues of Yellow-fleshed Cassava (Manihot esculenta Crantz) Root cv. Bocou 2

Aims: The objective of this work was to study the thermal inactivation of the polyphenol oxidase (PPO) and peroxidase (POD) activities of cassava (Manihot esculentaCrantz) root cv. Bocou 2 in order to preventenzymatic browning.Study Design: Crude PPO and POD from yellow-fleshed cassava root were subjected to heat treatment and their thermal inactivation characteristics were examined.Place and Duration of the Study: The study was conducted at Biocatalysisand Bioprocesses Laboratory, Food Science and Technology Unit, Nangui Abrogoua University Abidjan, Côte d’Ivoire, between January and December 2015.Methodology: The crude PPO and POD were extracted from three tissues (cortex, cambium and central pith) ofyellow-fleshed cassava root cv. Bocou 2. The thermal inactivation of these enzymatic activities was evaluated between 50 and 70 °C. The kinetic data of thermal inactivation and thermodynamics were analysed. Results: The t1/2-and D-values decreased with increasing temperature, indicating a faster inactivation of PPO and POD at higher temperatures. Z-values ranged from 16.10 to 27.70 °C and activation energy (Ea) from 73.37 to 129.66 kJ mol-1. Thermodynamic investigations indicated that the oxidation reactions involving these enzyme activities were: not spontaneous (?G > 0), slightly endothermic (?H > 0) and reversible (?S < 0).Conclusion: The PPO and POD activities from yellow-fleshed cassava root decreased due to heat denaturation with increasing temperature from 60 to 70 °C. These kinetic data can be used to prevent enzymatic browning in cassava roots.

Efecto de recubrimientos de almidón modificado de yuca, proteina aislada de soya y aceite esencial de orégano aplicados a la papaya / Effect of modified yuca starch coatings, isolated protein of soya and oregano essential oil applied to the papaya

RESUMEN La papaya tiene elevada demanda en el mundo; sin embargo, la producción y la exportación se afectan por microorganismos fitopatógenos y pérdidas pos cosecha, cercanas al 25-40% en la cadena de suministro, sobre todo, en transporte y en almacenamiento, debido a un deficiente manejo. La aplicación de recubrimientos comestibles permite mejorar el brillo y la textura de la corteza, reducir el deterioro de la calidad fisicoquímica y organoléptica, la pérdida de peso por deshidratación y el intercambio de gases. Por lo anterior, durante trece días, en papayas recubiertas con almidón de yuca modificado variedad SM 707-17 (4%), proteína aislada de soya (2%) y aceite esencial de orégano (250ppm y 500ppm), se evaluó el efecto sobre pérdida de peso, de color, de firmeza, de sólidos solubles, de respiración, de pH, de acidez titulable y de crecimiento del Fusarium spp., a condiciones ambientales, mediante un diseño completamente al azar con dos factores, tiempo y tratamientos. El experimento, se hizo por triplicado y los datos fueron sometidos a un análisis estadístico, utilizando el programa SPSS V.23. Los resultados indicaron que la utilización de almidón, por sí solo o combinado con aceite esencial de orégano (250ppm) y proteína aislada, lograron reducir la pérdida de peso, controlar la respiración al reducir el porcentaje de CO2 y retardar el desarrollo del color amarillo y rojo en las papayas frente al control, además de retrasar el crecimiento del hongo Fusarium solani; también, se encontró que los recubrimientos no incidieron sobre características fisicoquímicas, como el pH, la acidez titulable, los sólidos solubles y la firmeza de los frutos.

SUMMARY The papaya has a high demand in the world, however, its production and export are affected by phytopathogenic microorganisms and post-harvest losses close to 25-40% in the supply chain, especially in transport and storage due to poor management. The application of edible coatings improves the gloss and texture of the bark, reduces the deterioration of the physicochemical and organoleptic quality, the weight loss due to dehydration, and the exchange of gases. Therefore, for thirteen days, papayas were coated with cassava starch modified SM 707-17 variety (4%), isolated soy protein (2%) and oregano essential oil (250ppm and 500ppm). The effect was evaluated on weight loss, color, firmness, soluble solids, respiration, pH, titulable acidity and growth of Fusarium spp., to environmental conditions. A completely random design with two factors, time and treatments, the experiment was done in triplicate, and the data were subjected to a statistical analysis using the SPSS V.23 program. The results indicated that the use of starch alone or combined with essential oil of oregano (250ppm) and isolated protein were able to reduce weight loss, control respiration (CO2 percent) and slow down the development of the yellow and red color in the papayas against the control in addition to delaying the growth of the fungus Fusarium spp. It was also found that the coatings did not affect physicochemical characteristics such as pH, titulable acidity, soluble solids, and firmness of the fruits.

Powdered tucupi condiment: sensory and hygroscopic evaluation

Abstract Tucupi is a fermented liquid obtained from cassava (Manihot esculenta Crantz), very much appreciated by the traditional cuisine of Northern Brazil. However, there are no scientific reports on its use in the formulation of products. Thus, the present study aimed to elaborate a powdered condiment with tucupi, as well as to assess the product's sensory acceptability and its hygroscopic behavior. The powdered tucupi used in the formulation of the condiment was obtained by drying in a spray dryer. The product underwent sensory evaluation for its acceptability regarding the attributes of color, aroma, flavor, and overall impression and a purchase intention test was applied by hedonic scale. The acceptability index was 80% for overall impression and the purchase intention test indicated that 94% of the judges would be willing to buy the product. The product's moisture sorption isotherms presented type-II behavior for adsorption and type-III for desorption, at 25 °C. The hygroscopic behavior indicated that the product is more susceptible to spoilage changes when stored in an environment with relative humidity above 60% and the Peleg model showed an excellent performance on predicting the product's moisture sorption isotherms.

Salinity reduces nutrients absorption and efficiency of their utilization in cassava plants / Salinidade reduz a absorção e a eficiência de utilização de nutrientes em plantas de mandioca

ABSTRACT: The objective of this research was to evaluate the influence of salinity on the absorption and utilization of nutrients by cassava. For the study, cassava was submitted to four saline concentrations: 0, 20, 40, and 60mM NaCl. Results showed that the absorption of all nutrients, except nitrogen (N), was reduced by salinity, with highest reduction for potassium (K). However, all nutrients were maintained at concentrations which did not indicate mineral deficiency problem. The abnormal concentration of calcium in the tuberous roots may have been one of the factors that contributed to the lower growth of this organ and of the plant as a whole. Transports of nitrogen, potassium, phosphorus and sulfur from root to the aerial part was higher under salinity treatment. Efficiency in the use of all the nutrients, mainly N, was reduced due to salinity. Given that: (i) the absorption of K was the most impaired, (ii) there was abnormal accumulation of Ca in tuberous roots, and (iii) the efficiency in the use of N was the most affected, it is suggested to prioritize studies on these three issues, as a way to better understand the aspects related to the tolerance/sensitivity of cassava plants to salinity.

RESUMO: O objetivo deste trabalho foi o de avaliar a influência da salinidade sobre a absorção e utilização de nutrientes pela mandioca. Para o estudo, as plantas foram submetidas a quatro concentrações salinas: 0, 20, 40 e 60mM de NaCl. Os resultados mostraram que a absorção de todos os nutrientes, exceto o nitrogênio (N), foi reduzida pela salinidade, com maior redução para o potássio (K). No entanto, todos os nutrientes foram mantidos em concentrações que não indicaram problema de deficiência mineral. A concentração anormal de cálcio nas raízes tuberosas pode ter sido um dos fatores que contribuíram para o menor crescimento desse órgão e da planta como um todo. Os transportes de nitrogênio, potássio, fósforo e enxofre da raiz para a parte aérea foram maiores sob tratamento com salinidade. A eficiência no uso de todos os nutrientes, principalmente N, foi reduzida devido à salinidade. Considerando que: (i) a absorção de K foi a mais prejudicada, (ii) houve acúmulo anormal de Ca nas raízes tuberosas e (iii) a eficiência no uso de N foi a mais afetada, sugere-se priorizar estudos sobre estas três questões, como forma de melhor entender os aspectos relacionados à tolerância/sensibilidade das plantas de mandioca à salinidade.

Leaf area estimation of cassava from linear dimensions

ABSTRACT The objective of this study was to determine predictor models of leaf area of ​​cassava from linear leaf measurements. The experiment was carried out in greenhouse in the municipality of Botucatu, São Paulo state, Brazil. The stem cuttings with 5-7 nodes of the cultivar IAC 576-70 were planted in boxes filled with about 320 liters of soil, keeping soil moisture at field capacity, monitored by puncturing tensiometers. At 80 days after planting, 140 leaves were randomly collected from the top, middle third and base of cassava plants. We evaluated the length and width of the central lobe of leaves, number of lobes and leaf area. The measurements of leaf areas were correlated with the length and width of the central lobe and the number of lobes of the leaves, and adjusted to polynomial and multiple regression models. The linear function that used the length of the central lobe LA = -69.91114 + 15.06462L and linear multiple functions LA = -69.9188 + 15.5102L + 0.0197726K - 0.0768998J or LA = -69.9346 + 15.0106L + 0.188931K - 0.0264323H are suitable models to estimate leaf area of ​​cassava cultivar IAC 576-70.

Agronomic performance of cassava genotypes from the in vitro shoot tip culture submitted to clonal cleaning / Desempenho agronômico de genótipos de mandioca provenientes do cultivo in vitro de ápices caulinares submetidos à limpeza clonal

This study aimed to evaluate the agronomic performance of cassava genotypes from the in vitro shoot tip culture to eliminate the cassava frogskin disease for several root and aerial part characteristics. Cassava plants from accessions BGM0315, BGM0464 and BGM0841 infected with cassava frogskin disease were grown in a greenhouse after clonal cleaning. Cuttings from the three accessions were subjected to tetracycline concentrations (0, 5, 10 and 15 mg L-1) for three minutes, and then maintained in an acclimatized chamber (35 ± 1 °C and 16 hour photoperiod). Shoots were disinfected for excising shoot tips (0.2 mm and 0.4 mm) and inoculated in a culture medium containing the same concentrations of tetracycline used in the cuttings. After 60 days of cultivation, the explants were transferred to medium without antibiotic, 30 days later they were acclimatized for a period of 70 days for subsequent planting in the field. Seven months after planting, agronomical evaluation was held for root and aerial part characteristics. No influence of isolated shoot tip size was noticed on agronomic characteristics, while the addition of tetracycline in the culture medium, specifically at the concentrations of 5 mg L-1 and 15 mg L-1, was favorable to the development of the root system of plants in the field. The results revealed that the agronomic performance of cassava plants derived from in vitro cultivation are higher for the production of basic propagation material for the following production cycles, as well as root production for commercial use with subsequent generation of income.

Este trabalho teve por objetivo avaliar o desempenho agronômico de genótipos de mandioca provenientes do cultivo in vitro de ápices caulinares para eliminação da doença couro de sapo (Cassava frogskin disease, CFSD), em relação a diversas características de raiz e da parte aérea. Para isso, foram utilizadas plantas de mandioca dos acessos BGM0315, BGM0464 e BGM0841 cultivadas em casa de vegetação após a limpeza clonal. Inicialmente, foram utilizadas manivas de plantas adultas infectadas com couro de sapo. Essas manivas foram submetidas a concentrações de tetraciclina (0, 5, 10 e 15 mg L-1) por 3 minutos. Posteriormente, foram mantidas em câmara climatizada (35 ± 1°C e fotoperíodo de 16 horas). Brotos foram desinfestados para excisão de ápices caulinares (0,2 mm e 0,4 mm) e inoculados em meio de cultura contendo as mesmas concentrações de tetraciclina empregadas nas manivas. Aos 60 dias de cultivo, os explantes foram transferidos para meio sem antibiótico, 30 dias após foram aclimatizadas por um período de 70 dias para posterior plantio em campo. Aos sete meses após o plantio realizou-se a avaliação agronômica para características de raiz e parte aérea. Não houve influência do tamanho do ápice caulinar isoladamente nas características agronômicas avaliadas, enquanto que a adição de tetraciclina no meio de cultura, especificamente nas concentrações de 5 e 15 mg L-1, foi favorável ao desenvolvimento do sistema radicular das plantas no campo. Os resultados evidenciaram que o desempenho agronômico de plantas de mandioca provenientes do cultivo in vitro são elevados para produção de material propagativo básico para os ciclos seguintes de produção, bem como para produção de raízes para uso comercial com consequente geração de renda.

Estabilidade físico-química e microbiológica de farinha de mandioca fermentada comercializada em Belém do Pará / Physicochemical and microbiological stability of fermented manioc flour marketed in Belém do Para

A farinha de mandioca fermentada é um alimento típico da região amazônica brasileira e sua produção ocorre com as seguintes etapas: colheita, descascamento, fermentação (tubérculos são imersos em um tanque com água ou córrego), lavagem, trituração, prensagem, moagem e torrefação. A farinha de mandioca é uma fonte importante de carboidratos na Amazônia, especialmente pelas pessoas de baixa renda. Neste trabalho estudou-se o comportamento físico-químico e microbiológico da farinha de mandioca fermentada comercializada em um mercado de rua e em supermercado. Para tal, 36 quilogramas de farinha de mandioca fermentada foram adquiridos e mantidos sob as mesmas condições ambientais do mercado de rua e do supermercado. Durante dois meses, as amostras foram analisadas quanto à umidade, cinzas acidez total titulável, pH e atividade de água. Os resultados mostraram que o pH e a acidez total titulável foram estáveis, mas a umidade e a atividade de água mudaram e aumentaram significativamente (p>0,05).(AU)

High genetic diversity among and within bitter manioc varieties cultivated in different soil types in Central Amazonia

Abstract Although manioc is well adapted to nutrient-poor Oxisols of Amazonia, ethnobotanical observations show that bitter manioc is also frequently cultivated in the highly fertile soils of the floodplains and Amazonian dark earths (ADE) along the middle Madeira River. Because different sets of varieties are grown in each soil type, and there are agronomic similarities between ADE and floodplain varieties, it was hypothesized that varieties grown in ADE and floodplain were more closely related to each other than either is to varieties grown in Oxisols. We tested this hypothesis evaluating the intra-varietal genetic diversity and the genetic relationships among manioc varieties commonly cultivated in Oxisols, ADE and floodplain soils. Genetic results did not agree with ethnobotanical expectation, since the relationships between varieties were variable and most individuals of varieties with the same vernacular name, but grown in ADE and floodplain, were distinct. Although the same vernacular name could not always be associated with genetic similarities, there is still a great amount of variation among the varieties. Many ecological and genetic processes may explain the high genetic diversity and differentiation found for bitter manioc varieties, but all contribute to the maintenance and amplification of genetic diversity within the manioc in Central Amazonia.

Populational fluctuation of lace bug in cassava / Flutuação populacional do percevejo-de-renda em cultivares de mandioca

Vatiga illudens is a pest of economic importance for the cultivation of cassava. Knowing the time of incidence of this insect in combination with environmental factors enables control strategies. The objective of this study was to determine the population dynamics of V. illudens in cassava cultivars and the correlation of these insects for climatic factors in Dourados, Mato Grosso do Sul. Four cultivars of cassava were evaluated: Kiriris, N-25, IAC 90 and Fécula Branca. Population assessment of the lace bug was conducted over 18 months observing 2 crop cycles. Fortnightly, they were randomly sampled four central plants of each plot and five leaves from the middle third of each plant, quantifying the number of nymphs and adults per leaf. Twenty months after planting cassava, two central rows of each plot were harvested and measured productivity in kg ha-1. The experimental design was randomized blocks with four treatments and two replications. The peak population of nymphs and adults for the first crop cycle occurred during the months from March to May. For the second cycle, these population indices happened in the months from January to April. Cultivar Kiriris showed the highest mean number of nymphs and adults per leaf cassava and cultivar IAC 90, the lowest number of insects. There was a negative correlation between the population of adult insects and root yield. The maximum, mean and minimum temperatures affected the population of nymphs V. illudens. These results are fundamental to define management strategies for control of lace bug.(AU)

Vatiga illudens é uma praga de importância econômica para a cultura da mandioca. Conhecer a época de incidência desse inseto em associação com fatores ambientais possibilita estratégias de controle. O objetivo deste trabalho foi determinar a flutuação populacional de V. illudens em cultivares de mandioca e verificar a correlação desses insetos com os fatores climáticos em Dourados, Mato Grosso do Sul. Foram avaliadas quatro cultivares de mandioca: Kiriris, N-25, IAC 90 e Fécula Branca. A avaliação populacional do percevejo-de-renda foi realizada durante 18 meses, observando 2 ciclos da cultura. Quinzenalmente, foram amostradas aleatoriamente quatro plantas centrais de cada parcela e cinco folhas do terço médio de cada planta, quantificando o número de ninfas e adultos por folha. Vinte meses após o plantio, foram colhidas duas fileiras centrais de mandioca de cada parcela e mensurada a produtividade em kg ha-1. O delineamento experimental foi em blocos casualizados com quatro tratamentos e duas repetições. O pico populacional das ninfas e adultos para o primeiro ciclo da cultura ocorreu nos meses de março a maio. Para o segundo ciclo, esses índices populacionais aconteceram nos meses de janeiro a abril. O cultivar Kiriris apresentou maior número médio de ninfas e de adultos por folha de mandioca e o cultivar IAC 90, o menor número de insetos. Houve correlação negativa entre a população de insetos adultos e a produtividade de raízes. As temperaturas máximas, médias e mínimas afetaram a população de ninfas de V. illudens. Esses resultados são fundamentais para definir estratégias de manejo para controle do percevejo-de-renda.(AU)

Morpho-anatomy of leaves and yield of pineapple plant in intercropping with cassava / Morfoanatomia foliar e produtividade de abacaxizeiro consorciado com mandioca

The aims of this study were to evaluate the effects of shade in pineapple plant with cassava, on the yields of pineapple fruit sand cassava roots, on the morph-anatomical variation, damage by solar radiation and chlorophyll content of pineapple leaves. Pineapple plants were cultivated in plots comprising three parallel row sunder different shade conditions provided by cassava plants grown at various spacings within single rows located on either side of the plots. The experiment was of a randomized block design with five treatments (cassava spacings of 0.50, 0.75, 1.00 and 1.25 m and a pineapple monoculture) and four replications. Yields of fruits and roots, together with the morphological, chemical and histological characteristics of the "D" leaves of pineapple, were determined14 months after planting the pineapples plant. Data were submitted to analysis of variance with the Scott-Knott test or Friedman test (P< 0.05) and to regression analysis. Pineapple plants grown under the majority of shade conditions presented higher fruit weight and overall yield per hectare in comparison with plants grown in direct sunlight. The maximum productivities of pineapple fruit were achieved when cassava plants were spaced 0.75 m apart and, under these shade conditions, fruits were fully protected against burning by the sun. The "D" leaves of shaded plants were longer and thicker, with higher content of chlorophyll a and total chlorophyll, reduced stomatal density and dimensions of stomatal pores, narrower guard cells, thinner aquiferous hypodermis, and reduced abaxial and adaxial epidermis. The yield of cassava roots per plant increased linearly with increasing distance between the plants, but the yield per hectare decreased with decreasing plant density.

Os objetivos desse trabalho foram avaliar o efeito do sombreamento no abacaxizeiro com mandioca, sobre a produtividade de abacaxi e raízes de mandioca, sobre a variação morfoanatômica, queimadura do abacaxi pela radiação e os teores de clorofilas nas folhas do abacaxizeiro. As plantas de abacaxizeiro foram cultivadas em parcelas compreendendo linhas triplas paralelas (segundo um arranjo triangular) sob diferentes condições de sombreamentos proporcionadas por plantas de mandioca cultivadas em linhas simples em cada lado das parcelas. O delineamento utilizado foi de blocos casualizados com cinco tratamentos (sombreamento com mandioca em espaçamentos de 0,50, 0,75, 1,00 e 1,25 m e monocultivo de abacaxi) e quatro repetições. As produtividades de abacaxi e mandioca, juntamente com as características morfológicas, químicas e histológicas da folhas "D" dos abacaxizeiros, foram determinadas 14 meses após o plantio. Os dados foram submetidos à análise de variância com teste de Scott-Knott ou teste de Friedman (P< 0,05) bem como análise de regressão. Abacaxizeiros cultivados sob sombreamento produziram frutos com maior peso médio e maior produtividade por hectare em comparação com plantas cultivadas sob luz solar direta. A máxima produtividade de abacaxi foi alcançada quando o espaçamento entre as plantas de mandioca foi de 0,75 m e, sob essas condições, os frutos ficaram completamente protegidos da radiação solar. As folhas "D" dos abacaxizeiros sombreados foram mais longas e espessas, apresentaram maior concentração de clorofila a e total, densidade estomática reduzida, poros estomáticos menores, células guarda mais estreitas, hipoderme aquífera mais fina e epidermes abaxial e adaxial reduzidas. A produtividade de mandioca por planta aumentou linearmente com o espaçamento crescente, porém a produtividade por hectare diminuiu com o decréscimo da densidade das plantas.